Metagenomic Diagram

First, we start with the isolation of bacteria, then we extract its DNA, after it we digest it using restriction enzymes to insert it into a plasmid BAC vector, once ligated we electroporate the cell to insert the plasmid+insert into the cell and finally we screen the transformants.

Photo from: Handelsman, J., Rondon, M., Brady, S., Clardy, J. and Goodman, R. 1998. Molecular Biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem. Biol. 5: R245-249.